Acrylamide induced histochemical and immunohistochemical alterations in rat kidney cortex

Document Type : Original Article

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Histochemistry and Cell Biology Department Medical Research Institute, Alexandria University, Egypt

Abstract

Acrylamide represents an industrial chemical and has become one of the main public health concerns since it was detected in extensively consumed food items. The present study was planned to investigate the effects of two doses of acrylamide on some enzyme activities and immunoreactivity of some immunohistochemical parameters in kidney cortex of male rats. Rats were randomly divided equally into three groups. Group (I) was control, group (II) was given acrylamide for 6 months orally in a dose of 0.05 mg/ /Kg dissolved in water for 3 doses per week and group (III) was administered acrylamide in a dose of 0.5 mg by the same way as in group II. At the end of the experiment all animals were sacrificed under anesthesia, kidney was immediately removed and processed for histochemical and immunohistochemical studies. The results revealed that the activities of lactic dehydrogenase (LDH) and xanthine oxidoreductase (XOR) were significantly increased, while succinic dehydrogenase (SDH) activity was decreased significantly compared to control group. On the other hand, immunohistochemical results showed that acrylamide significantly reduced the immunoreactions of endothelial nitric oxide synthase (e- NOS) in addition to a significant increase in immunoreactivity of inducible nitric oxide synthase (i- NOS) and insignificant increase in alpha smooth muscle actin (α-SMA) with respect to control group. Overall, these results suggested that the deleterious effect in kidney tissue resulted from oral administration of acrylamide, most probably due to oxidative stress and lipid peroxidation

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