fertility in induced azoospermic mice

Impairment of spermatogenesis has been shown in patients treated with chemotherapy against different malignancies resulting in azoospermia. The present study was designed to assess the ability of anti-SCa-1and CD105 mesenchymal stem cells to restore fertility in induced azoospermic mice following cyclophosphamide administration. Forty male mice were divided randomly into four groups, each group included 10 male albino mice which has an average body weight 22-25 g; group (1): normal control, group (2): male azoospermic mice induced with cyclophosphamide, group (3): male azoospermic mice received anti-SCa-1 + stem cells into caudal vein, group (4): male azoospermic mice received CD105 MSCs into caudal vein. Androgen hormonal profile and histological assessment of testicular tissues were evaluated pre- and post-treatment with anti-Sca-1 + and mesenchymal stem cell transplantation. Results showed that male azoospermic mice treated with anti-SCa-1+ and CD105 MSCs stem cells after 12 weeks of treatment are able to improve androgen hormonal profile levels and resuming spermatogenesis as verified by histological assessment of testicular tissues and hormonal profile as compared to control groups. Also data obtained from the study showed no significant differences between anti-SCa-1+ and CD105 MSCs treated groups. In conclusion, both of anti-SCa-1 and CD105 MSCs have the potentiality to differentiate into germ cells and sperms in vivo in testicular microenvironment, and also able to restore testicular functions.