Oral supplementation of aqueous Ginkgo biloba extract inhibits oxidative stress and hippocampus injury associated with methotrexate injection.

Document Type : Original Article

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Zoology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt

Abstract

Methotrexate is effective for the treatment of a number of cancers including breast, lung, leukemia an osteosarcoma. This study was conducted to examine the possible modifying effects of Ginkgo biloba against hippocampal and brain toxicity induced by methotrexate in male albino rats. This could be fulfilled through the histological, immunohistochemically and biochemical analysis of hippocampal brain tissues. The experiments were performed on 36 male albino rats divided into 6 groups; the first and second groups were the control and Gingko groups respectively while the 3rd group methotrexate rat group; the 4th and the 5th groups were co- and post treated Methotrexate rat groups with Gingko respectively and the 6th group was methotrexate self-treated rat group. In brain homogenates Malondialdehyde (MDA) levels in methotrexate group showed a significant increase when compared with control group, in contrast methotrexate-treated group also exhibited a significant decrease in brain antioxidants machinery represented by catalase, reduced glutathione (GSH) and total proteins. The histological changes consisted of destruction of oligodendrocytes, sometimes complete over large areas, and sometimes relatively slight. All the rats which survived long enough after treatment showed severe astrocytosis. Rats’ brains immunocytochemical results showed that GFAP-positive cells astrocytes increase after Exposure to (MTX) compared with the other groups.

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