Using starchy waste as a promising raw material for bioethanol production with consequence purification using chitosan/sodium alginate polymeric membrane

Document Type : Original Article

Authors

1 Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt.

2 Polymer Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt.

3 Chemistry Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

4 Environment and Natural Materials Research Institute (ENMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egypt

5 Environment and Natural Materials Research Institute (ENMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab, 21934, Alexandria, Egyp

Abstract

The current study is concerned with using environmental wastes as raw materials for bio-ethanol production. The alpha-amylase enzyme has been used to convert the starch molecules of kitchen waste into simple units of glucose which are subsequently fermented into bioethanol. The obtained results showed that 40% substrate and 100 µl of α-amylase were the optimum concentration to produce the highest glucose units at 417.9 and 482.5 milligram/deciliter (mg/dl), respectively. The highest bioethanol production of 423.5 mg/dl was obtained after anaerobic fermentation of free yeast cells at 30oC without shaking. Both bio-ethanol and 25% ethanol were separated by using an amicon cell ultra-filtration system integrated with chitosan or sodium alginate membranes under nitrogen pressure. Each membrane was characterized by Scanning Electron Microscope (SEM) and Ion Exchange Capacity (IEC); while, the hydrophilicity/hydrophobicity was investigated using contact angle. The whole system succeeded to elevate the ethanol concentration in a range of 47-50%, which could be increased with more polymeric modifications

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