Induction of renal cell line apoptosis by hydrogen peroxide

Alhalfawy, Khalil A.; Elfiky, Bahgat A.; Zahran, Ahmed M.; Zayed, Mahmoud M.

doi:10.21608/jbaar.2022.228865

Induction of renal cell line apoptosis by hydrogen peroxide

Document Type : Original Article

Authors

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

² Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute – University of Sadat City, Egypt

³ Internal medicine and nephrology Department, Faculty of medicine - Menoufia University, Egypt

10.21608/jbaar.2022.228865

Abstract

Background: Hydrogen peroxide is currently the most widely used as an apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types. cytotoxicity is achieved over a wide range of doses
Objective: To evaluate the cytotoxic effect of hydrogen peroxide on renal cell lines by detecting RIPK1.
Methods: In this study, we used a Vero cell line treated with H₂O₂ at concentrations 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM, and 1.6 mM and examined after 30 min,1hour,2hours,3hours, 4hours and 5 hours. by using MTT assay to detect cytotoxicity to cell line (by detecting cell viability). Spectrophotometrically measure the absorbance at a wavelength of 570 nm. Measure the background absorbance of multi-well plates at 690 nm and subtract from the 450 nm measurement. Sub-lethal dose to renal cell line is one treated with 1.6 mM for 5 hours. groups group 1: renal cell line as control which not treated by H2O2. group 2: Sub-lethal which renal cell line treated by concentration 1.6 mM of H2O2 for 5 hours. Results: H2O2 is cytotoxic to renal cell line by concentration from 0.1 mM to 1.6 mM. RIPK1 gene expressed in renal cell line treated by H2O2. The sublethal dose reached 1.6 mM for 5 hours. There is a significant difference between the 2 groups by detecting the expression of the RIPK1 gene.

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