Exploring the Biophysical Mechanisms of Taurine's Effect on Myeloperoxidase Enzymatic Kinetics in Pre-Diabetic and Type 2 Diabetic Patients

Muneam, Suha A.; Muneam, Nada. A.; N., Mihad Shakir

doi:10.21608/jbaar.2023.331983

Exploring the Biophysical Mechanisms of Taurine's Effect on Myeloperoxidase Enzymatic Kinetics in Pre-Diabetic and Type 2 Diabetic Patients

Document Type : Original Article

Authors

¹ Department of Chemistry and Biochemistry, Al-Iraqia University/ College of Medicine -Baghdad, Iraq.

² Department of Physiology and Medical Physics, Al-Iraqia University/ College of Medicine -Baghdad, Iraq.

³ Department of Physiology, Al-Iraqia University/ College of Medicine -Baghdad, Iraq

10.21608/jbaar.2023.331983

Abstract

Background: investigate the enzymatic activity of Myeloperoxidase (MPO) in pre-diabetic and diabetic individuals and explore the modulation of this activity by taurine supplementation, considering its potential anti-oxidative properties and the emerging evidence of its role in glucose metabolism.
Methods: This case-control study was done at the Iraqi University College of Medicine. It used advanced spectroscopic techniques and kinetic modeling to measure the amount of MPO activity in the sera of people who were healthy, pre-diabetic, and diabetic. The Lineweaver-Burk plot derived from the Michaelis-Menten equation was used to ascertain the Km and Vmax of MPO. Taurine inhibition assays were also performed to understand its effect on MPO kinetics.
Results: The data showed that MPO activity went up significantly from the control group to the diabetic group. This was in line with rising HbA1c levels and BMI, suggesting a link between MPO activity, glycemic control, and obesity. The gender distribution showed no significant deviation, suggesting that the observed enzymatic and metabolic alterations are not gender biased.
Conclusion: The pronounced elevation in MPO activity in diabetic individuals underscores the enzyme's potential significance in glycemic diseases. The results mean that more research needs to be done on how taurine, which is known to have anti-inflammatory and antioxidant properties, could improve MPO activity and possibly restore metabolic homeostasis, opening a new way to treat type 2 diabetes.

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