Determination of ex vivo chemical and in vivo biological antioxidant activities of clary sage essential oil

Document Type : Original Article

Authors

1 Science department, College of Basic science, Misan University, Misan, Iraq

2 Department of Biology, College of Education for Pure Science, University of Basra, Basrah, Iraq.

Abstract

Several studies have shown that clary sage (Salvia sclarea) oils or extracts can prevent and treat several health conditions. Clary sage oil was tested for antioxidant and anti-cancer effects on HCAM cell line development and integrity. GC-Mass. examined SAGE oil chemical components The oil's free radical-scavenging ability was tested using DPPH. Sage oil's biological influence on cancer cell formation and progression was examined by measuring antioxidant indicators SOD and CAT at different time intervals. The oil’s antiproliferative capacity on cancer cells (toxicology test) was also assessed on HCAM by using the method of MTT viability test. According to the results of the GS-MS analysis, the procedure was successfully yielded 100% total identification of the chemical composition of Clary sage oil, of those are Gamma-terpinene (17.847%), heptane (16.759%), D-Limonene (12.654%), isopropyl myristate (10.179%), and linalool (8.189%). The examined antioxidant activity showed increase in the level of catalase enzyme at 1.5h followed by complete reduction at 72h post treatment. SOD was also reduced significantly after 72 h incubation and was not observed to be affected by exposure to oil. The oil showed cytotoxic activity (IC50=17.32µg/ml) compared to control, and HCAM cell line genomic DNA integrity was degraded on gel. These data suggest Clary sage oil may be an antioxidant and anti-cancer agent. This study emphasizes the relevance of shorter sage oil exposure intervals for optimal pathophysiological signals against hepatocellular carcinoma progression.

Keywords

Journal of Bioscience and Applied Research

Articles in Press, Accepted Manuscript
Available Online from 21 February 2024

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