Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections
Background: Urinary tract infections (UTIs) frequently affect the urinary system, predominantly caused by Escherichia coli (E. coli) and Klebsiella pneumoniae. This study examines the prevalence of these bacteria and bla OXA-48 and bla IMP genes. Objectives: The research aims to assess the occurrence of MDR and XDR E. coli and K. pneumoniae in pediatric UTI cases, including the resistance genes bla OXA-48 and bla IMP. Methods: A total of 100 pediatric UTI urine samples were analyzed for antibiotic resistance, ESBL detection, and bla OXA-48 and bla IMP gene presence using molecular techniques, gene sequencing, and statistical analysis via SPSS software. Results: High resistance rates to common antibiotics were found in K. pneumoniae and E. coli isolates. bla OXA-48 was detected in 92.3% of E. coli and 100% of K. pneumoniae isolates, while bla IMP was found in 43.1% of E. coli and 25.7% of K. pneumoniae isolates. No nucleic acid variation was observed in the blaIMP-1 sequences of both bacteria. Conclusion: The significant presence of bla OXA-48 and bla IMP genes in K. pneumoniae and E. coli highlights the urgent need for enhanced surveillance and careful antibiotic use to curb antibiotic-resistant UTIs in children.Bottom of Form
Ahmad, N., Wamidh, Q., & Abdulrahman, T. (2024). Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections. Journal of Bioscience and Applied Research, 10(1), 85-102. doi: 10.21608/jbaar.2024.348053
MLA
Noor Ahmad; Qudus Wamidh; Thanaa Rasheed Abdulrahman. "Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections". Journal of Bioscience and Applied Research, 10, 1, 2024, 85-102. doi: 10.21608/jbaar.2024.348053
HARVARD
Ahmad, N., Wamidh, Q., Abdulrahman, T. (2024). 'Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections', Journal of Bioscience and Applied Research, 10(1), pp. 85-102. doi: 10.21608/jbaar.2024.348053
VANCOUVER
Ahmad, N., Wamidh, Q., Abdulrahman, T. Detection of bla OXA-48 and bla IMP Resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from Children with Urinary Tract Infections. Journal of Bioscience and Applied Research, 2024; 10(1): 85-102. doi: 10.21608/jbaar.2024.348053