Characterization of Paramyrothecium roridum isolated from soil

Document Type : Original Article

Authors

1 Department of biology

2 College of Science, Mosul University, Iraq

3 Northern Technical University, College of Agricultural Technology, Plant Production techniques Mosul, Iraq

Abstract

The fungus Paramyrothecium roridum (Tode) L. Lombard and Crous, previously known as Myrothecium roridum Tode ex Fries, belongs to the family Stachybotryaceae, the species of Myrothecium distributed worldwide in the soil and decaying plant tissues as saprophytic fungi or as pathogens on diverse hosts. The morphology of asexual morph, particularly the characteristics of conidia and conidiophores, was previously used to classify species in this genus, because few features distinguish species within a genus, morphology-based identification alone is inaccurate; consequently, DNA sequence data is useful in identifying species. The fungal isolate was identified according to morphological characteristics. In PDA culture, its olive green conidia were 6-6.5 μm long × 2.3 μm wide, and cylindrical with rounded ends. Conidia formed dark green to black masses on sessile sporodochia in concentric zones, as well as identified based on the molecular approach by using polymerase chain reaction assay of internal transcribed spacer (ITS1 5.8S ITS2) of the rDNA to confirm of Paramyrothecium roredium by using primers (ITS1 and ITS4), the produced band size was 600 bp. The nucleotide sequences of amplification products were analyzed, the results confirmed Paramyrothecium roredium diagnosis. As a results, the isolate Paramyrothecium roridum fungus was recorded as JFF22 isolate in National Center for Biotechnology Information (NCBI) for the first time in Iraq.

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