Estimate MFE of secondary structure and thermodynamic Ensemble prediction of hsa-mir-181a ACE2 in un/vaccinated samples compared with the wild type

Document Type : Original Article

Authors

1 Department of Pathological Analyses, Faculty of Science, University of Kufa, Iraq

2 Department of Medical Laboratory Technology, Faculty of Medical Technology, University of Imam Jaafar Sadiq, Najaf, Iraq

3 Department of Biology, Faculty of Science, University of Kufa, Iraq

Abstract

Using intelligent computer programs, the secondary structure and thermodynamic properties of hsa-mir-181a ACE2 were investigated in both vaccinated and unvaccinated samples, comparing them to the wild type. By utilizing the RNAalifold web server, a consensus structure of aligned micro-RNA sequences was predicted, along with the minimum free energy (MFE) and equilibrium base-pairing probabilities. The results showed a strong correlation between the secondary structure of hsa-mir-181a ACE2 and the vaccination status, with a higher MFE observed in the vaccinated group. This study proved a significant relationship between the secondary structure of hsa-mir-181a ACE2 and the selected class of vaccinated or unvaccinated. The value of (MFE) of the secondary structure of microRNA increased in vaccinated compared with unvaccinated, with a significant difference evident. Also, at the thermodynamic free energy (value) level, the vaccination was increased compared to the unvaccinated as the difference was clear and the relationship was achieved.

Keywords

Journal of Bioscience and Applied Research
Volume 10, Issue 5 - Serial Number 5
Special issue: The Third International Scientific Conference for Pathological Analyses College of Science, University of Basrah, Iraq February 14 – 15, 2024
December 2024
Pages 169-179

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