Expression of TNF-α miRNA 155 in Eris (EG.5) patients associated with toxoplasmosis

Document Type : Original Article

Authors

1 Middle Technical University/ College of Health and Medical Technology, Iraq

2 Middle Technical University/ Kut technical institute, Iraq

Abstract

In this case-control study, 100 patients infected with Eris were recruited to Al-Numan Hospital in Baghdad City, and 50 healthy people were also selected as a control group during the period from July 2024 to August 2024.
The results showed that the males who were infected with Eris (EG.5) were higher 52 (52.0 %) than females 48 (48.0%) with non-significant variation (P=0.81). The results also demonstrated there were equal cases of Eris (EG.5) 50 (50 %) recorded in the urban and rural areas with a non-significant difference (P= 0.87). The levels of Toxo-IgM IU/ml were higher among patients infected with Eris (EG.5) (2.78±6.69) than the control group (0.07-0.15), with highly significant variation  (p≤0.0001). The levels of Toxo-IgG IU/ml were higher in patients infected with Eris (EG.5) (2.12±3.13) than the control group (0.08 ±0.15) with highly significant variation (P≤0.0001). The levels of IL-1β pg/ml were higher in infected patients (24.21±8.39) than the control group (5.03±2.65) with highly significant variation (P≤0.0001), and the levels of IL-10 pg/ml were higher in infected patients (10.42±2.42) than the control group (4.05±2.02) with a highly significant difference (P ≤0.0001). The levels of TNF-α pg/ml were higher in infected patients (18.58±8.23) than the control group (4.65±1.91) with highly significant variation  (P≤0.0001). The Ct value of  miR155 of control groups was (18.63±0.17), while the Ct value of patients group was (18.57±0.24), with no significant difference. The computed ratio for gene fold expression was 3.46 for the patients group and 1.46 for the control group.     

Keywords

Journal of Bioscience and Applied Research
Volume 11, Issue 1 - Serial Number 1
January- February- March
March 2025
Pages 337-345

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