Antibacterial Effect of Natural Honey of known active components by GC-MS technique against Staphylococcus aureus

Document Type : Original Article

Authors

1 Medical Laboratory Techniques Department / Mosul Medical Technical Institute / Northern Technical University, Mosul, Iraq

2 Department of Medicinal Plant Technologies, Technical Agricultural College, Northern Technical University, Iraq

Abstract

Background: Staphylococcus aureus is a human pathogenic bacterium that colonizes the skin, oral cavity, upper respiratory tract, and gastrointestinal tract. It causes skin, soft tissue infections, and hospital-acquired infections. Aim: isolating Staph. aureus from skin infection and using honey as an alternative pathway to therapeutic therapeutic-resistant bacteria causing skin infection, as well as estimating the active compound by using the GC-MS Technique. Methods: (110) Clinical samples were collected from patients with skin infections through consultation clinics at Ibn Sina General Hospital of Mosul City/ Iraq, during the period from September 2024 until December 2024, by using conventional methods and the VITEK 2 system for identification. Result: The rate of isolation of Staph. aureus from skin infection was 76 (69.09%), but at the age group (11-30) years was 25% and (30-50) years was 40.7%, (51-70) years at 22.3% while at ages less than 10 years was 6.5% and ages more than 70 years was 5.2%. This bacterial species showed resistance against most of the 10 antibiotics under test using the disk diffusion method. Whole honey with its four dilutions (25,50,75, and 100%) gave an antibacterial effect against Staph. aureus growth. Gas Chromatography Mass Spectrometry (GC-MS) was used to investigate the biological effectiveness of honey and its (11) components, which have antibacterial effects.

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