Cytotoxicity Activity of Doxorubicin, Tamoxifen Drug, and n-Hexane Annona squamosa Extract Against Cancerous Cell Line (MCF-7 and SKOV-3) and Normal Cell Line NHF

Document Type : Original Article

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Department of Biology/ Biotechnology, College of Science, University of Kufa/, Iraq

Abstract

Herbal medications are usually regarded as an alternative treatment option for cancer. Many novel cytotoxic chemicals have been identified from the plants that are used to fight cancer. The current research determined the cytotoxicity of Annona squamosa extract (ASE). Different cancer cell line (MCF-7 and SKOV-3) and normal cell line with different concentrations (1000, 500, 250, 125, 62.5 and 31.25) as fallow G1 (n-Hexane Annona Squamosa extract, HAS), G2 (doxorubicin, DOX), G3 (Tamoxifen, TAM), G4 (HAS+DOX, HDA), (HAS + TAM, HTA). Each inhibitory agent group was used to treat the cancer cell lines MCF-7, SKOV-3, and NHF to obtain IC50% by MTT assay. In 1000 µg/ml from HAS showed significantly increased all three cell lines compared with other inhibitory concentrations. The recorded data demonstrated that the inhibitory ability of each of HTA and HDA towards the MCF-7 significantly increased compared with the SKOV-3 cell line. The interaction between different concentrations of DOX and TAM showed a high inhibitory effect on MCF-7 compared with SKOV-3 cancerous cell lines. The HAS and TAS showed a significant effect on MCF-7 compared with SKOV-3. Both HAD and HTA showed antagonistic activity on normal cell lines, while HAD and HTA showed synergistic activity towards MCF-7 and SKOV-3.

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